Currently Being ModeratedMar 8, 2012 5:42 AM (in response to wildscorp)
Probably not as this error is usually caused by another piece of software on your system such as AntiVirus/security software that is configured to scan your iPod. Then next time this happens, try to simply reboot your PC to see if that does any good. If not, temporarily disable all AntiVirus and security software to see if that helps.
Currently Being ModeratedDec 28, 2012 2:25 PM (in response to wildscorp)
Go to your computer's System Tray and try ejecting. If you can't eject, there is an application that is trying to access your iPod.
Go to http://technet.microsoft.com/en-us/sysinternals/bb896653.aspx and download ProcessExplorer.
Go to http://support.microsoft.com/kb/555665 and follow the instructions under Resolution.
Note that this is a good, general process any time you can't access any file.
In my case an update to RealPlayer installed a program called RecordingManager that was accessing my iPod.