I changed it somehow! I can't remember how I did it?
I have the keyboard/character monitor in the top right but am fed up of dragging it into my tweets, how do I set the shortcut back to alt+shift+3?
Go to system prefs/language & text/input sources and make sure that only the box for the keyboard you want is checked. Could it be British, where # is alt+3 (not alt+shift+3)? US is shift+3.
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