There are four general causes of this issue:
1. The computer's PRAM no longer contains a valid startup disk setting when there aren't any problems with the disk itself. This can be checked for by pressing the Option key and seeing if the drive appears.
2. The internal drive's directory structure has become damaged. This requires usage of an alternate bootable system to perform the repair.
3. Critical system files have been deleted. This requires usage of an alternate bootable system to reinstall them.
4. The internal drive has died or become unplugged. This is the most likely case if the computer took a sharp impact or there are unusual sounds coming from the hard drive's location.
And if the little box in the center of the screen shows up and is blinking means that your Hard Drive has crashed. Or at least that was my expieriance when I had one shoot craps. Niel of course has much more expieriance than I do.