By startup disk, it means your hard drive that has Mac OS X installed. So just take some files and trash them.
Of course, don't trash these or their contents:
the System folder
the Library folder (whether it's in your home folder or sitting next to your System folder)
any app in the Applications folder that came with the Mac (unless it's some app like Quicken) (Microsoft Office you'll have to run the uninstaller if you want to uninstall it)
To find big files to delete, use something like
GrandPrespective.
And if there's one file that looks WAY too big, reply about it.