Do not know what happened but I replied to this a couple of hours to ago as the same thing happened opening up this morning. However just got another one.
I was in Time Machine Backup trying to delete some old unwanted files and when I closed out; Cancel; up pops the error message for my backup disk. What is really funny is the icon was still on the desktop and worked properly when double clicked. Just to be sure Ii checked the USB connection and all seemed to be ok/tight. As a final check, I did a restart and the error message did not show up. So, am not really confused as to what is happening.
Also, just to clarify, I only saw the error messages the first two times but totally ASSUMED it was for the USB Drive. Meaning those could have been that I had somehow loosened the USB for my backup and when I checked them reseated it properly as everything SEEMS normal now.
Sparkgapper