Problem with cron which run python script for detecting position of mouse.

When using cron, make sure that cron has permissions:

Cron needs full disk access and automation to run certain things in the background.

Full Disk Access:

Go to "System Preferences" on your macOS.
Select "Security & Privacy."
Go to the "Privacy" tab.
In the left sidebar, select "Full Disk Access."
Click the lock icon in the lower-left corner to make changes and enter your administrator password.
Drag your terminal application or the script interpreter into the list to grant it full disk access.

Automation:

Look for "Automation" in the left sidebar.
Check if your terminal or script interpreter is listed and has access to the necessary applications or services.

I had this trouble a while back with using cron. Once I put cron in those two boxes, my cronjob started working. There are other things within the privacy and security tab to be tweaked, but that depends on what your script does and requires.

I hope this helps you out.

Maybe you need to update your sudoers file to allow this script to run with admin privileges since you said it worked fine when you called it with sudo. Maybe call it from cron with the sudo command, after you've edited the sudoers file and added the script to run with admin privileges. Maybe that would change things up for you.