This app installed on my computer while I was installing something else. Brand new Mac, suddenly, I have a piece of software running that I didn't want? Not cool.
So, I moved the Tune-Up program to the trash from Applications, but when I tried to Empty my Trash, the system (10.7.5) came back with App In Use, can't delete.
I went to Tune-Up's site, as suggested by Patrick, and followed their instructions:
http://support.tuneupmedia.com/entries/414968-how-can-i-uninstall-tuneup-from-my -computer
This got rid of all the hidden folders and files, but the App was still running, and Trash wouldn't delete it.
I finally found a solution:
I opened Activity Monitor under the Utilities Folder in Applications, found the Tune-Up processes in the list (there were two running), highlighted them, then click Quit Process.
Once the program was no longer running, Empty Trash was successful in deleting the file.