How to Validate Transparent Alpha Channel in .mov File?

I'm trying to create transparent flv files to embed in Abobe Captivate.

I shot and keyed some test greenscreen (yes, I have the checkerboard in the canvas) and have tried exporting it a number of ways (rendered/unrendered, recompressed all frames/not, etc, etc) and converted it to flv a number of ways (change mov to flv, encoding software), but when I embed it and play it back I have a black background.

How can I validate that my .mov file contains the alpha channel and it's transparent?

Second, when the .mov is correct, will changing .mov to .flv keep the transparent alpha channel or do I need us encoding software with a codec that supports transparent alpha, e.g., On2 V6?

I'm pulling my hair out here...