Turn off the startup chime
Killing the infernal BONG on my mid 2011 iMac with 10.7 was easy- hit mute before shutting down. If you have something more-or-less permanently plugged in to the audio-out (headphone) port as I do be aware that you must unplug it, wait a sec for the machine to switch to internal speakers and then hit mute to silence the speakers. This setting will "hold" after plugging something back into the headphone port. There is no reason to go to the output pane of sound preferences because there is no "switch" there- just an indicator telling you whether the machine is sending to internal speakers or the headphone port. The switch is made by plugging something into the headphone port.