RE: Mac Pro 65 lb tower computers ONLY:
Mac Pro uses ECC memory 72 bits wide. Apple-specified Heatsink and temperature-reporting chip is REQUIRED, and is especially necessary for denser modules.
2008 and previous: Uses dual-channel. Matched pairs are required, all slots populated is optimum. (less than all populated is slightly slower). DIMMs are plugged into Riser cards. All the riser cards you will ever need should have shipped in your Mac.
2009 and later: Triple-channel memory is used. Modules in slot 3 and 4 (on each side, if dual processor chips) are shared. Three DIMMs is optimum, two is an estimated five percent slower, four is a little more than five percent slower, but more than compensated by having more RAM available. Largest DIMMs go in the lowest-numbered slots (towards the door in the first set). No riser card is used -- the processor(s) and RAM is on a large daughter card (on its own removable shelf).
--------
The way you asked your question makes me think you probably have a great new replacement memory that is working perfectly, but is the wrong memory for your Mac.
If you deal only with Vendors who know Macs, you tell them your model and they send you the proper RAM and the proper number of modules for your proposed expansion, "Guaranteed to work in your Mac or your money back!"