NON system / apps moved to another drive,
You can for convenience leave ~/Library which is your home account library which is about 1GB
leave that stuff where it is, and move the system to another drive.
use it as is for a backup for now
Your system uses then about 250GB total - there has to be 40GB or more just for the system to play with, to burn a DVD, temp files and other things.
Boot drive
audio libraries
projects and media files
scratch
backup
... that would be a common setup for storage.
OS X can and does often use 1GB for kernel, Safari can take up as much. But some of the way a modern OS works is if you give it more, if it has more RAM or memory to work with, it will. So sometimes you can go from 3GB to 6GB and find it using as much as it can grab and then some.
OS X and some programs have had trouble releasing pages from memory even after closing windows, quitting apps.
In some cases repair permissions will force pages to be freed, in other cases only more RAM and sometimes a restart. Varies by program.
I was looking for a recommended minimum, and then for what people actually find is needed for best - maybe the Pro apps forums like the one for Logic Pro and others on Apple Community. And depend on particular users and projects of course as to how many concurrent tracks and processes that they tend to work with.