Well, a standard "main" router that will supply DHCP and NAT services for the entire network will be able to allow up to a total of about 250 devices to connect. That would include the AirPorts, and any devices that are connected to the AirPorts and any other devices that you have on the network.
Each AirPort Express (new version) will handle up to 50 wireless clients. It would be much better to limit that to 20-25 to keep the speed up. So, you'll have to do some math to try to figure out how many total devices will connect to the network when everything is working.
You could connect 20 AirPort Express devices to the main router....but you would only be able to connect about 10-15 devices to each Express.
Or, connect 10 Express devices to the main router and you can connect about 20-25 devices to each Express.
If you plan to connect to the Internet, keep in mind that all devices will share the bandwidth. If you have a 100 Mbps Internet connection, and 100 clients connected to the network, then each client will get about 1 Mbps. Pretty slow.
I'm not sure what your audio application is here....the network examples I am providing assume that you will be connecting wireless and wired Ethernet clients to the network.
If you could detail your proposed system, other users could offer their comments. We're still pretty much guessing at hypotheticals at this point.