My MBP 2010 with 16GB of Ram will not boot up.

Have you tried to switch the 8 GB models between the banks as well (not just test both on the same bank)? Do you hear beeps upon start up with both 8 GB modules installed? If so, describe.

See this information from everymac.com on your machine. 16 GB is not officially supported by Apple:

*Originally, both the official and actual maximum RAM was 8 GB. However, as confirmed by site sponsor OWC, if running OS X 10.7.5 or higher, updated with the latest EFI, and equipped with proper specification memory modules, this model can support up to 16 GB of RAM.

http://www.everymac.com/systems/by_year/macs-released-in-2010.html

Additionally, I personally would not buy Kingston RAM (especially Value RAM) as there have been quite a few reports that it is not working here. As Macs are rather finicky about their RAM, I would only buy RAM from a reliable vendor with a lifetime warranty, specifically one of the two most recommended here: OWC (macsales.com) or Crucial.

OWC has tested it with 16 GB - see the notes here:

If I wanted to go beyond Apple's specs, the only place I'd feel comfortable buying from would be OWC since they'll guarantee it.

I'd return the RAM and purchase it from one of the two recommended vendors.

Let me echo the advice that babowa has offered.

Macs are notorious for being selective with RAM. OWC has bench tested 16 GB RAM that they sell in a MBP model 7.1. I know of no other vendor that has done that (though other RAM brands MAY work).

For guaranteed results in a model 7.1 MBP with 16 GB RAM, send your current RAM back and purchase OWC RAM. Otherwise, you will have to be satisfied with your current arrangement.

Ciao.

I am currently working with One 8GB and the orginal stock 2GB, Is this slower and worse off?

Yes it is. The computer will benefit from a matching RAM kit (same capacity in both slots, i.e. 2 x 4 or 2 x 8 GB).