My iphone 5s Bluetooth no longer works in my 2014 honda accord, ever since i updated the software two months ago. Can anyone help?

Problems with Bluetooth connections are generally traced back to the vehicle. If you search here in the forum you will find a number of posts with issues connecting with Honda vehicles. Try removing the phone from the vehicle's list of Bluetooth devices. Then try removing the vehicle from the iPhone's list of Bluetooth devices. Then place both devices back in discovery/pairing mode and try to connect them. You may need to take the device to the dealer to check for software updates.

Next, I understand it used to connect, however software updates for the iPhone will load the latest Bluetooth stack into the phone and vehicles do not always get updated as often. You can also check this support document for frequently asked questions and the other for additional troubleshooting instructions. Wireless input devices: Bluetooth frequently asked questions iOS: Third-party Bluetooth accessories