I'm a big audio nerd and I listen to a lot of more
complex/higher-range music. So I've converted nearly
all of my physical CD Library to Lossless in iTunes,
from MP3 320K originally.
I definitely do notice a difference.
Most people wouldn't consider a 10 fold increase in
file size worth it for the ability to hear a little
more backgound noise but I certainly do.
I agree on the importance of the subtleties, and for me it really is a matter of if I can discern any difference at all. I am not so sure that complexity of music is a factor, because I find I often notice differences most in simple acoustic recordings of folk music, and also in old recordings where the original is poor already and you can't afford to lose any more.
I have been using iTunes for a long time and take my music from the computer to my stereo as my primary music source. I have never liked the quality of iTunes Store music, nor any other downloaded music for that matter. I never understood the popularity of Napster because I consider music from there to be impossible to enjoy for quality reasons quite aside from the legal aspects.
So my ears thank you for your input here
I will be concentrating on whether or not I can tell the difference between 320 AAC and Apple Lossless on the iPod both with headphones and with the stereo. I have seen discussions and reviews on classical music sites where it is claimed that experienced classical listeners cannot tell the difference between high bit rate AAC and the original CD on an iPod. I remain to be convinced. My first experiments are that there is a big difference that I can notice, not in the main body of the music, but in the subtle noise in the background as you describe, but which is important to me, as it appears to be for you.