Folder with a question mark
If you see a folder with a question mark appear instead of the Apple logo, it means your Mac couldn't find a local or network-based startup disk. This can happen if the disk selected in the Startup Disk pane of System Preferences isn't available. Wait a few seconds to see if your Mac is able to locate the startup disk you specified.
If you still see a question mark after waiting a few moments, use Startup Manager to start up your Mac, then re-select your startup disk from System Preferences.
If a question mark appears after you install a software update, re-select your startup disk using OS X Recovery.