If you are being billed for Spotify through iTunes, see the following: View, change, or cancel your subscriptions - Apple Support
To delete the app, press and hold the app icon on the home screen until all the apps start jiggling, then tap the x in the corner of the Spotify icon and then press the home button to stop the jiggling. Note that deleting the app will not cancel your subscription. You need to do that separately.
If you are unsure what iTunes is charging you for, see: iTunes Store charges on bank, credit, and debit card statements - Apple Support