There has to be more to this question that you've asked because the simplest answer is to move the folder out of the DocumentRoot. That way no one can access it.
However, I suspect what you really want is to
restrict access to it, either to certain users, or to certain IP addresses or some such, correct?
If that's the case the specifics will vary depending on precisely which access controls you want to implement (e.g. username/password, IP address, and so on.
The official word on Apache authentication can be found at
http://httpd.apache.org/docs/1.3/howto/auth.html, but it' a lenghty document. If you can be more specific about what you're trying to do I'm sure someone can post a more specific answer.