Has the low resolution mode option been removed?

Okay, I checked one more Mac. It did exist in Big Sur so apparently it has been removed in Monterey. I couldn't find it because the Macs I checked with Big Sur or earlier didn't have a Retina display. Duh me. lol

In addition to needing a 5k or retina display, it is not enabled for all apps, mostly graphics, photo and video apps. So depending upon what type of programs you are using, you might not even see the option to open in low resolution mode, but if you;re running Photoshop, Lightroom, Capture One, etc. it is there and VERY beneficial.

In Terminal try:

sudo nano /Applications/VirtualBox.app/Contents/Resources/VirtualBoxVM.app/Contents/Info.plist

Change this

<key>NSHighResolutionCapable</key>          <true/>

To this  or add if missing

<key>NSHighResolutionCapable</key>          <false/>

Or in one big command

sudo /usr/libexec/PlistBuddy \
    -c 'set NSHighResolutionCapable false' \
    /Applications/VirtualBox.app/Contents/Resources/VirtualBoxVM.app/Contents/Info.plist

Indeed, editing the .app/Contents/Info.plist is the way to go.

In the case of HOROS (a DICOM viewer for medical imaging), one has to introduce the following:

	<key>NSHighResolutionCapable</key>
	<false/>

Please note that 'nano' (in the terminal) and TextEdit fail to open .plist files properly. The best option is to use BBEdit (works in evaluation mode).

Greetings from Athens.

Still appears on my late-2015 5K iMac.

That screenshot does not apply to Monterey. This is on a 2017 iMac 5K screen:

What were the conditions under which your screenshot was taken? Mac model type, year, graphics card, etc.

You don't see where it says (low resolution)? Do you need a larger image?

Got it. It's been so long since I've seen that setting. Even High Sierra 10.13 no longer used it.

Do you know which version of macOS/OS X that was?

I'll have to take your word for it because it not on any of my Macs that I checked; Monterey, Big Sur, Catalina or High Sierra.