Same issue here too... good to know I'm not the only one.
M1 MackBook Pro running Mac OS 12.0.1 on M1 Max chip
Intel MacBook Pro running Mac OS 11.6 on Intel i7 chip
I use After Effects to generate animated lower thirds. I then use them in Wirecast as part of live streams. I noticed that when i upgraded to my new M1 MacBook Pro that all the backgrounds on my lower thirds were no longer transparent, but black. The exact same video files would however show transparency on my old intel MacBook Pro.
When running them in Finder as a preview (select file and press Space bar) on both old and new MacBooks I get the same results.... transparent backgrounds on the old intel, black background on the new M1.
I did a test and outputted a video with a straight alpha channel, and another with a premultiplied alpha channel. Neither worked on the new M1 (still black background), but both worked on the old Intel MacBook Pro.
All my videos are being outputted as .mov file using Apple ProRes 4444 as the codec. QuickTime shows that these files have alpha channels in Video Details.
I gave the videos files to my business partner to check too on his M1 MacBook Pro and he got the same results, black background.
So only difference between my two macbooks is OS, and chipset.
I'm reluctant to update my old Intel MacBook Pro to Mac OS 12 in case I loose the ability to run videos with transparency as this is now my back up lpatop for live events that require it. Really had hoped to switch fully over to the new M1 but now I carry two laptops around!
So having not resolved the issue, I'm here to ask if anyone else has?