If you're talking about one of the OWC black eSATA enclosures like this one...
http://eshop.macsales.com/item/Other%20World%20Computing/MESATATBE/
and intend to use it as an eSATA RAID....a few caveats.
Firstly, you will have to get a PCI dual-port eSATA controller card, not a single port. When you install the drives, each drive has a SATA cable connecting to it, but not the other. Meaning that each of the two ports on the back are for one drive each, not daisy-chaining...it's effectively two independent drives in one enclosure, not one big one combining the two. What's important about this, aside from the connections, is that you'll be running a SOFTWARE-based RAID, not a HARDWARE-based one in which the casing's internals handle the striping. This will be controlled by your computer...meaning extra work for your computer....and you'll need to get the right drivers to do that. There could be a lot of guesswork with MAC compatability when it comes to those eSATA cards. It will be the computer handling the striping to the two drives, or whatever RAID configuration you want to set up.
Secondly...if you look at the included cables/connectors, you'll notice the two power connectors. Those are the older SATA II (or whatever) power connections, which don't come on all the recent internal drives from WD/Seagate or what have you. Notice the small power connectors on your Mac's own internal drives? If you got a pair of those, that don't include the older/larger power connectors, you won't be able to use them in that OWC enclosure.
So beware. I was looking into this, but really didn't fancy the idea of having to set up a software RAID, so I just went with the Lacie Two Big, which is a true hardware RAID and came with its own eSATA card and drivers for Mac.
PowerMac G5 Mac OS X (10.4.3) Dual 1.8