Yes, by adding the Macintosh HD then removing it simply toggling Spotlight on/off. This is the most user friendly way to toggle Spotlight. You can also do this in the Terminal, but that scares some users.
How to reset Spotlight using the Terminal
Copy the text below and open Terminal (in /Application/Utilities). Paste the code into the Terminal window at the prompt.
sudo mdutil -E /
You will be asked for your administrator password (and if you've never used the sudo command you'll need to accept the warning presented). When you type your password, it will look like nothing is being typed. That's normal. Once you've typed your administrator password, press Return.
This will completely remove the Spotlight index and force a new one to be created. Probably not necessary, but I would immediately quit Terminal and restart your Mac.
SUMMARY:
Depending on your Mac and the contents of it's hard disk, re-indexing may take several hours. Doing this overnight is a good option if you have a large drive.
You can tell if it's still in progress by simply looking at the Spotlight menu: There's a blinking dot in the middle of the magnifying glass while indexing is occurring. You can also open Activity Monitor in Applications/Utilities and type in md in the search filter. You should mdworker. If you have Microsoft Outlook you'll also see mdworker32.
Until this process is finished, Spotlight won’t know the contents of your disks and neither will Mail.