OK, this is interesting. More and more of these Apple Sierra network issues are becoming clear.
After the Sierra update, Sophos prevented networked time machines from working, both for Apple devices and other vendors which have been implement the protocol across the last 4 OS X releases. I also noted an oddity here where Apple Sierra was struggling with standard AFP network paths. for example, /Volume/home to a network ceased working. By looking in /Volumes with ls -la I was surprised to see that Sierra had a /Volumes/home-1 path which was working, but that the /Volumes/home, while displayed, was non functional. At that time I noted that Sierra clearly had some issues in the networking area. Also, networked Time Machines were now failing with Sierra whereas they continued to function perfectly with El Capitan machines.
Then I had a minor Sierra update and voila... suddenly Time Machine began functioning again. Then, while running some weekly scripts which I had modified AFP paths to home-1 accommodate to Sierra's AFP networking bugs, I noticed that they were no longer functioning. It turns out that the Sierra 10.12.1 update corrected the newly introduced underlying AFP bugs which impacted both AFP and Time Machine access. The home-1 path is gone.
So, get Sierra 10.12.1. Apple has fixed the Sierra network layer and both the Time Machine protocol and AFP network paths function properly at this point. Obviously there was some major rewriting in this area or it would not have made it past a beta. I imagine that this has caused some work for Sophos and it does explain the concerns which folks have raised here that Sophos should have caught this during the beta. I'm a long time distributed systems architect and developer and many of you use my products every day if you've ever so much as touched your money electronically over a network, so I know of what I speak.