Recovering Permanately Deleted Photos from iPhone

Hello! I'm looking for some guidance. I recently plugged my iPhone 6S Plus into my computer and transferred some pictures directly off of my phone and onto a flashdrive. The photos never made it to a file on my PC and iTunes was not open. After transferring the photos, I then unplugged the flashdrive and deleted the photos off of my phone, through file explorer on my PC. So, of course, the pictures never went to the recently deleted file in my Photos. The problem that I am having is that one picture never made it to the flashdrive. I accidentally overlooked it while transferring, and then deleted it off my phone... Not knowing that I didn't have a copy on my flashdrive. I'm looking for a way to recover this picture, and I'm not sure where to start. I already checked my backups, and the last backup I have was from before I took the picture(I have a bad habit of not backing up my phone), and I didn't have iCloud set-up at the time of the picture. Any advice would be greatly appreciated. Thank you!!